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. 2013 Dec 6;192(1):234–244. doi: 10.4049/jimmunol.1301302

FIGURE 7.

FIGURE 7.

Neutrophil GP degrade LLO. (A) LLO (38 nM) was incubated alone (0), with increasing amounts of GP (11, 44, and 88%, v/v, indicated by the wedge) or heat-inactivated GP (HI; 88%, v/v) for 1 and 5 min at 37°C. (B) LLO (38 nM) was incubated in the absence (−) or presence (+) of GP and PI cocktails that contain EDTA (PIEDTA) or not (PI) or EDTA alone for 5 min at 37°C. (C) LLO (38 nM) was incubated for 5 min at 37°C alone (−) or in the presence of GP (+) that were preincubated with α2-macroglobulin (α2-m), PIEDTA, or PI. (A–C) Samples were analyzed by Western blotting. Full-length LLO is indicated by an arrow. Shown are representative results from at least three independent experiments.