FIG. 4.

Anti-integrin antibodies inhibit the binding of FMDV to β8-transfected SW480 cells. β8-transfected cells (SW480αvβ8) were incubated with antibodies to αvβ5 (P1F6), αvβ8 (37E1), α5β1 (SAM-1), or αv (L230) at 6 μg/ml prior to the addition of virus (O1Kcad2; 10 μg/ml), and the cells were analyzed for bound virus by flow cytometry (see Materials and Methods). Data are expressed as percentages of the level of binding in the absence of the competing antibody (set at 100%) and are means of two independent experiments, each carried out using triplicate samples, that gave near-identical results.