FIG. 5.

Binding of FMDV to β-transfected SW480 cells is inhibited by RGD peptides. β-transfected cells expressing wild-type αvβ8 (SW480/αvβ8) (A), wild-type αvβ6 (SW480/αvβ6) (B), or chimeric αvβ6/8 (SW480/αvβ6-8) (C) were incubated with an RGD-containing peptide (VPNLRGDLQVLA [solid bars] or GRGDSP [open bars]) or the control RGE version (VPNLRGELQVLA [hatched bars] or GRGESP [checked bars]) prior to the addition of FMDV O1Kcad2 (10 μg/ml). Cell-bound virus was detected by flow cytometry using the anti-FMDV antibody B2 followed by an R-phycoerythrin-conjugated goat anti-mouse IgG1 antibody. Data are means from two independent experiments, each carried out by using triplicate samples, that gave nearly identical results.