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sgC1qR induces the expression of B1R. ECs were grown for 24 h, as described Fig. 1, until full attachment and cobblestone formation were achieved. The cells were then cultured or not with 5 μg/ml gC1qR for an additional 24 h. The expression of B2R or B1R was examined by incubation of the cells with the corresponding Abs and analyzed by deconvolution immunofluorescence microscopy. Original magnification ×68.
