FIG. 1.

CD46-expressing cells bind Ad3. (A) Flow cytometry of human leukemia K562 cells and rodent BHK cells that were not incubated with (white histograms) or that were incubated with (shaded histograms) Ad3- Alexa-488. (B and C) Single-cell sorting of BHK cells infected with Sindbis virus expressing a K562 cDNA library (B) and control Sindbis virus (C) after staining with propidium iodide (PI), anti-Sindbis virus antibodies (FL4), and Ad3- Alexa-488 (FL1). (D) PI-negative, Sindbis virus- and Ad3-positive cells (gate) were single cell sorted into 24-well plates containing BHK feeder cells and rescreened for Ad3- Alexa-488 binding. One of 30 positive clones is shown.