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Western blot analysis of supernatant from rMd5/ΔvIL-8-RV (lane 1), rMd5/ΔvIL-8 (clones 1 and 2 in lanes 2 and 3), rMd5 (lane 4), rMd5/vIL-8-ELR (clones 1 and 2 in lanes 5 and 6), and mock infected DEF (lane 7) using anti-vIL-8 rabbit polyclonal sera. The vIL-8 protein is about 18 kDa.
