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. 2004 May;78(9):4753–4760. doi: 10.1128/JVI.78.9.4753-4760.2004

FIG. 3.

FIG. 3.

Southern blot analysis of DNA isolated from recombinant MDVs. (A) Viral DNA was digested with EcoRI and probed with all five radiolabeled cosmids. (B) Viral DNA was digested with BamHI (lanes 1 to 6) or double digested with BamHI/SalI (lanes 1′ to 6′) and hybridized with the 3.1-kb MDV BamHI fragment containing the vIL8 gene. Lanes: 1 and 1′, uninfected DEF; 2 and 2′, rMd5; 3, 4, 3′, and 4′, rMd5/vIL-8-ELR (clones 1 and 2); 5, 6, 5′, and 6′, rMd5/ΔvIL-8 (clones 1 and 2). BamHI single digestion produces a 3.1-kb band in both rMd5 and rMd5/vIL-8-ELR viruses and a 2.3-kb fragment in rMd5/ΔvIL-8 virus. BamHI/SalI double digestion results in two bands (1.8 and 1.3 kb) in rMd5 and a single band (3.1 kb) in rMd5/vIL-8-ELR, due to loss of the SalI site.