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Immunohistochemical analysis of FFE cells from inoculated chickens. FFE cells were sampled at 2 weeks postinoculation. All of the recombinant viruses rMd5 (A), rMd5/ΔvIL-8 (B), and rMd5/vIL-8-ELR (C) expressed viral antigen in FFEs, indicating that the second lytic infection is not impaired in either the vIL-8 gene deletion or vIL-8 gene mutations. No viral antigen was detected in the control chickens (D).
