Skip to main content
. 2013 Dec 31;11(12):e1001743. doi: 10.1371/journal.pbio.1001743

Figure 4. NRG does not affect neuronal activity.

Figure 4

(A) Lucifer yellow filled patch-clamped interneuron, identified after recording by labelling for glutamate decarboxylase (GAD) 65/67, from which action potential evoked synaptic currents were recorded. DAPI labelling shows cell nuclei. (B) Spontaneous synaptic currents at −64 mV recorded in an interneuron with ECl = 0 mV. (C–D) Event frequency (C) and block by 1 µM TTX (D) in control and NRG cultures. (E) Spontaneous synaptic currents at −70 mV recorded with ECl = −88 mV, during application of 25 µM NBQX+50 µM D-AP5, or 10 µM GABAzine, or 1 µM TTX. (F–G) Event frequency (F) and block by NBQX/AP5, GABAzine, and TTX (G) in control and NRG cultures.