FIG. 4.
RNA dimerization properties of patient-derived HIV-1 leader RNA from early and late stages of infection. (A) Nondenaturing gel showing the dimerization properties of early (p95) and late (p99A and p99B) patient-derived HIV-1 leader RNA in the context of 1-290 and 17-290 transcripts. Arrows indicate the dimeric (D) and monomeric (BMH and LDI) RNA. (B) RNA dimer yield for the 1-290 transcripts shown in panel A. (C) RNA dimer yield for the 17-290 transcripts shown in panel A. (D) Analysis of the LDI-BMH equilibrium of the LAI and patient-derived leader RNA. Nondenaturing gel showing the LDI and BMH conformations of the LAI, p95, p99A, and p99B transcripts (positions 1 to 290). (E) Ratio of BMH to LDI conformers as determined by quantification of the gel shown in panel D.