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. 2004 May;78(9):4566–4572. doi: 10.1128/JVI.78.9.4566-4572.2004

FIG. 3.

FIG. 3.

Effects of proteasome inhibitors on HBV gene expression. HepG2 cells were infected with recombinant adHBV at a MOI of 5 and treated with two kinds of proteasome inhibitors, epoxomicin (EPOX) or MG132, at different doses (lactacystin was also tested for HBV transcription). As a control, cells were also treated with ALLM. The cells were harvested 2 days postinfection. (A) Effects on HBV replication. The core-associated DNA was extracted for the detection of replicative intermediates by Southern blotting with an HBV-specific probe. HBV Marker is the 3.2-kbp HBV genome digested with NcoI (1.8- and 1.4-kbp fragments). (B) Effects on HBV transcription. Total RNA was extracted for the detection of viral transcripts by Northern blotting. The 28S and 18S RNAs were shown as loading controls. (C and D) Effects on viral antigen production. The culture medium was tested for HBV surface antigen (HBsAg) (C) and HBV e antigen (HBeAg) (D). Results are representative of three independent experiments.