FIG. 5.

Effects of proteasome inhibitors on HBV replication in Huh7 cells. Huh7 cells were infected with the recombinant adHBV wild-type or X-minus mutant at a MOI of 5 and treated with three kinds of proteasome inhibitors, lactacystin (LC), epoxomicin (EPOX), or MG132, at different doses. As a control, cells were also treated with ALLM. The cells were harvested for viral infection 2 days postinfection. The core-associated DNA was extracted for detection of replicative intermediates by Southern blotting with HBV-specific probe. HBV Marker is HBV DNA/NcoI fragments. Results are representative of three independent experiments.