Table 1. Encystation markers identified at 0, 2, 4, 6, 8, 10, 12 and 14hpie.
| Total number of peptides | Protein up | Transcript up | ||||||||||
| ORF | Annotation | 0 hpie | 2 hpie | 4 hpie | 6 hpie | 8 hpie | 10 hpie | 12 hpie | 14 hpie | 2D PAGE | Microarray | SAGE |
| GL50803_112103 | Arginine deiminase | 93 | 98 | 104 | 86 | 84 | 102 | 123 | 115 | Y | - | - |
| GL50803_17063 | Pyruvate-flavodoxin oxidoreductase | 89 | 54 | 96 | 74 | 84 | 96 | 94 | 88 | Y | - | - |
| GL50803_103676 | Alpha-tubulin | 85 | 74 | 86 | 89 | 96 | 94 | 117 | 117 | Y | - | - |
| GL50803_10311 | Ornithine carbamoyltransferase | 77 | 53 | 65 | 70 | 74 | 88 | 104 | 121 | Y | - | - |
| GL50803_88765 | Cytosolic HSP70 | 76 | 82 | 82 | 91 | 87 | 99 | 106 | 118 | y | - | - |
| GL50803_98054 | Heat shock protein HSP 90-alpha | 37 | 28 | 36 | 40 | 36 | 40 | 41 | 46 | y | - | - |
| GL50803_15409 | Kinase, NEK | 34 | 28 | 34 | 30 | 35 | 36 | 38 | 42 | y | - | - |
| GL50803_12102 | Elongation factor 1-gamma | 31 | 30 | 32 | 28 | 37 | 35 | 33 | 42 | y | - | - |
| GL50803_7532 | Vacuolar ATP synthase catalytic subunit A | 21 | 21 | 22 | 22 | 19 | 22 | 21 | 16 | y | - | - |
| GL50803_4812 | Beta-giardin | 18 | 24 | 23 | 26 | 28 | 26 | 45 | 39 | y | - | - |
| GL50803_9115 | Glucose-6-phosphate isomerase | 11 | 12 | 11 | 16 | 20 | 23 | 27 | 25 | - | y | - |
| GL50803_4197 | Phosphatidylinositol transfer protein alpha isoform | 7 | 8 | 7 | 9 | 8 | 7 | 11 | 12 | - | - | y |
| GL50803_6184 | Branched-chain amino acid aminotransferase lateral transfer candidate | 3 | 2 | 7 | 3 | 5 | 4 | 4 | 3 | y | - | - |
| GL50803_102813 | Protein 21.1 | - | 6 | 5 | 4 | 5 | 4 | 5 | 8 | - | y | - |
| GL50803_5435 | Cyst wall protein 2 | - | 2 | 3 | 7 | 20 | 21 | 25 | 26 | - | y | y |
| GL50803_8172 | Dynein heavy chain | - | 2 | 3 | 2 | 3 | 3 | 2 | 3 | - | - | y |
| GL50803_8245 | Glucosamine-6-phosphate deaminase | - | - | - | 7 | 7 | 13 | 39 | 35 | - | - | y |
| GL50803_16069 | Phosphoacetylglucosamine mutase | - | - | - | - | 3 | 5 | 5 | 4 | - | y | y |
| GL50803_40376 | High cysteine non-variant cyst protein | - | - | - | - | 2 | - | 2 | 2 | - | - | y |
| GL50803_7982 | UDP-glucose 4-epimerase | - | - | - | - | 6 | 11 | 7 | - | - | y | |
| GL50803_2897 | Furin precursor putative serine protease | - | - | - | - | - | 2 | 4 | 5 | - | - | y |
| GL50803_16424 | Hypothetical protein | - | - | - | - | - | - | 4 | 3 | - | - | y |
| GL50803_16217 | UDP-N-acetylglucosamine pyrophosphorylase | - | - | - | - | - | - | 2 | 6 | - | - | - |
| GL50803_14626 | Oxidoreductase, short chain dehydrogenase | - | - | - | - | - | - | 2 | 2 | - | y | - |
| GL50803_8377 | Hypothetical protein | - | - | - | - | - | - | 2 | - | - | - | y |
Total number of identified peptides for 25 known encystation protein markers over the 14 hour encystation time-course experiment. CWP2 (GL50803_5435) and members of the GalNAc biosynthesis pathway (ORFs GL50803_ 9115, 16069, 7982, 16217 and 8245) are highlighted in orange. A “y” for “yes” marks ORFs previously reported to be induced by encystation in 2D PAGE [22], microarray [7] or SAGE experiments [19]. hpie: hours post induction of encystation.