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Expression and purification of soluble S1190 glycoprotein. Codon-optimized S1190 glycoprotein was cloned into pcDNA3.1 Myc/His and expressed in HEK-293T/17 cells to a level of 5 mg/liter. Protein was purified using metal-affinity chromatography, dialyzed, and concentrated. Purified protein was analyzed by Coomassie staining (A) or Western blotting using the anti-c-myc antibody for detection (B).
