FIG. 5.

Expression and binding of C-terminally truncated S proteins. (A) DNA encoding C-terminally truncated S proteins (S₃₅₀, S₄₉₀, S₅₉₀, S₆₉₀, and S₇₉₀) was synthesized via PCR using the vector encoding the S₁₁₉₀ glycoprotein as template. PCR products were cloned into pcDNA3.1 Myc/His and expressed in 293T cells. Western blotting was performed using the anti-c-myc antibody for detection. (B) Vero E6 (black bars) or HEK-293T/17 (gray bars) cells were incubated with a 100 nM concentration of each soluble S glycoprotein fragment and detected with antibody directed against the c-myc tag. FACS analysis was performed, and mean fluorescence intensity was plotted.