FIG. 8.
S270-510 binds to Vero E6 cells. (A) Schematic representation of the S510 and S270-510 glycoproteins. The black box (amino acids 1 to 14) represents the S glycoprotein leader sequence. (B) Vero E6 cells or HEK-293T/17 cells were incubated with S350, S590, or S270-510 at concentrations ranging from 0.1 nM to 1 μM. Binding of S glycoproteins was detected using the anti-c-myc antibody followed by anti-mouse IgG conjugated to PE. FACS analysis was performed, and mean fluorescence was plotted as a function of S glycoprotein concentration.