Figure 7. ChIP-seq Data from C2C12 cells demonstrate MyoD binding to the region deleted in Δ4b.

(A) Publicly available ChIP-seq data for MyoD and Myogenin in C2C12 cells were mapped to the first intron of UCP3. Interestingly, only one of these experiments demonstrates MyoD binding to the promoter of UCP3. Furthermore, ChIP-seq data for the co-activator p300 and RNA polymerase 2 were mapped. Screenshot taken from the ENCODE browser. ChIP-seq data for BAT was not available. (B) Alignment of the three intronic binding elements in hamster (Psu), rat (Rno) and mouse (Mmu). Intronic sequences were obtained from ENSEMBL (www.ensembl.org). Putative binding elements are marked by boxes. The fourth row of sequence resembles the Phodopus reporter gene construct carrying the deletion Δ4b. Shown are 25 bp of 36 bp deleted in Δ4b, but not in Δ4a. Numbers in brackets denote bases left out for the sake of clarity.