Table 1. Primers used for TD-PCR.

Primer	Type	Target region	Sequence (5′-3′)	Anneal temp (°C)
251F	Forward primer	5′ end of transposon	AACCGGAACCCATCGGCATC	62.89
251R	Reverse primer	3′ end of transposon	CGGAACCGCTGCTCCATACC	63.25
74F	Forward primer	Upstream of transposon	CACCTTGACCACGGGTTCGG	63.33
74R	Reverse primer	Downstream of transposon	CCAAAGCGGTATCTGCCGTGA	63.02