FIG. 5.

Antibody responses before and after MPOV challenge. (a) Sera from monkeys vaccinated with 4pox DNA vaccine, L1R DNA vaccine, negative control (Neg. cont.) DNA vaccine, or Dryvax were collected 1 week before MPOV challenge, at the time of challenge (day 0), and at the indicated times after challenge. PRNT 80% neutralization titers are shown. (b) Immunogen-specific antibody titers in sera collected during the week before challenge were determined by ELISA using partially purified A27L, L1R, B5R, or A33R protein expressed in E. coli. Each bar represents the geometric mean titer obtained from at least two experiments. (c) MPOV-specific neutralizing antibody titers in sera collected at the time of MPOV challenge (day 0). The bars represent the mean value of two to four determinations ± standard deviation.