Table 2. Thermodynamic parameters obtained for TPR-Hsp interaction using isothermal titration calorimetry.
Systems | N | ΔG (kcal/mol) | ΔH (kcal/mol) | TΔS (kcal/mol) | Kd (µM) |
T_C70_C5 | 0.93+/−0.13 | −4.37 | −1.63 | 2.72 | 568 |
T_C70_C8 | 1.2+/−0.12 | −4.9 | −1.0 | 3.86 | 230 |
T_C90_C5 | 1.04+/−0.13 | −4.17 | −2.68 | 1.47 | 800 |
T_C90_C8 | 0.94+/−0.04 | −4.94 | −6.18 | −1.26 | 218 |
N is the stoichiometry; ΔG is the calculated change in Gibb's free energy; ΔH is the change in enthalpy; ΔS in the change in entropy and Kd is the binding affinity.