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. 2013 Dec 31;8(12):e83703. doi: 10.1371/journal.pone.0083703

Figure 2. Binding of His6-EF-Tu to sulfated glycolipids assessed by SPR analysis.

Figure 2

(A) Binding of His6-EF-Tu to sulfatide (SO3-3Galβ1Cer) at different pH values (pH 4.0, 5.0, 6.0, and 7.2). (B) Binding of His6-EF-Tu to various glycolipids: sulfatide, sulfated-lactosylceramide (SO3-3Galβ4Glcβ1Cer), galactosylceramide (Galβ1Cer), lactosylceramide (Galβ4Glcβ1Cer), and GM3 (NeuAcα3Galβ4Glcβ1Cer) at pH 5.0 and 7.2. Resonance units were measured at the start of dissociation. Error bars indicate standard deviations (n = 5). (C) Sensorgrams of the interaction of His6-EF-Tu with sulfatide at pH 5.0. Concentrations of His6-EF-Tu (from top to bottom) are as follows: 350, 300, 250, 200, and 150 nM. The KD value is described in the text.