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. 2013 Dec 31;8(12):e83703. doi: 10.1371/journal.pone.0083703

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© 2013 Nishiyama et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Supernatant, cell surface, and whole cell lysate fractions at different culture times were analyzed by western blotting. Outer surface proteins of L. reuteri JCM1081 were extracted with GHCl. Reactivity with anti-EF-Tu and anti-RNA polymerase antibodies is shown. An anti-RNA polymerase antibody was used to confirm whether cell lysis occurred. Culture time and pH are indicated in the table.