FIG. 7.

Western blots analysis. Lysates were prepared at the indicated hours postinfection (h.p.i.). In the presence or absence of PAA, Vero cells were either mock-infected or infected with either KOS or the polymerase null mutant virus HP66. As a control for DNA damage, mock-infected cells were also treated with 1 μM camptothecin (CPT) for 6 h prior to harvest. (A) Membranes were probed with antibodies directed against UL29, NBS1, Ku86, RAD51, and RPA32 as described in Materials and Methods. The open arrows indicate the slower-migrating species of NBS1 and RPA32. Results for tubulin are provided as a loading control. (B) Membranes were probed with antibodies directed against RAD52, RAD50, MRE11, and DNA-PKcs.