Table 1. List of Angiogenic genes identified and pairwise comparison of gene expression between gestational age groups.

Gene Identifiers		E14.25 vs E15.25		E15.25 vs E17.25		E17.25 vs E20		E14.25 vs E20
Gene name	Entrez ID	Fold change	p value	Fold change	p value	Fold change	p value	Fold change	p value
Up- regulated 22 genes
Bmp4	25296	–	ns	–	ns	3.8	***	6.77	***
Vegfa	83785	–	ns	<2	*	–	ns	3.04	***
Mmp14	81707	–	ns	<2	***	–	ns	3.44	***
Acvrl1	25237	–	ns	2.8	***	–	ns	3.42	***
Edn1	24323	–	ns	–	ns	<2	***	3.61	***
IL18	29197	–	ns	–	ns	6.4	***	6.74	***
Gpx1	24404	–	ns	–	ns	3.7	***	3.69	***
Id1	25261	–	ns	–	ns	<2	***	4.27	***
Cd36	29184	–	ns	2.8	***	<2	***	4.87	***
Hs6st1	316325	–	ns	–	ns	<2	***	<2	***
Emcn	295490	–	ns	<2	**	<2	***	2.94	***
Rhob	64373	–	ns	–	ns	<2	***	2.31	***
Prl7d1	84377	–	ns	–	ns	<2	***	9.29	***
Angptl4	362850	–	ns	3.1	***	3.7	***	6.93	***
Sox18	311723	–	ns	<2	**	–	ns	<2	***
Klf5	84410	–	ns	<2	*	<2	**	<2	***
Pf4	360918	–	ns	–	ns	3.0	***	2.38	***
Cav1	25404	–	ns	6.7	***	<2	***	15.46	***
Sgpl1	286896	–	ns	–	ns	<2	***	2.64	***
Notch4	406162	–	ns	–	ns	<2	***	<2	***
Tek	89804	–	ns	<2	*	2.2	***	4.72	***
Tgm2	56083	–	ns	<2	**	<2	***	2.34	***
Down-regulated 9 genes
Itgb2	309684	–	ns	–	ns	>0.5	**	>0.5	***
ItgaV	296456	–	ns	–	ns	>0.5	*	0.41	***
Itga4	311144	–	ns	>0.5	*	0.4	***	ns	***
Foxm1	58921	–	ns	>0.5	**	0.4	***	0.20	***
Anpep	81641	–	ns	–	ns	0.2	***	0.2	***
Gbx2	114500	–	ns	–	ns	0.2	***	0.13	***
Atg5	365601	–	ns	>0.5	**	–	ns	>0.5	***
Cited2	114490	–	ns	–	ns	0.5	***	0.5	***
Nos2	24599	–	ns	>0.5	*	0.2	***	0.16	***

E14.25 vs E15.25 - no significant differences in gene expression. E15.25 vs E17.25–15 of 31 genes were differentially expressed. E17.25 vs E20–26 of 31 genes were differentially expressed. E14.25 vs E20 shows gene expression changes for each gene from E14.25 vs E20. Significance was ascribed where gene expression was changed by <2 (* = p<0.05, p<0.0005 = **; p<0.0001 = *** and ns = p>0.05). The order of the genes listed in the table corresponds to the Heat Map on Figure 2.