Table 1. Primer used in RACE of siRNA components of whitefly.
5′ RACE (5′-3′) | 3′ RACE (5′-3′) | ||
Dicer2 | GSP1 | GTTGATCGAACTGCTGATTCCAC | GGAAGGAGCAGCCTCAGGGTT |
NGSP1 | CTTACAGCTTGCGCCTGTTGCTC | CGGGAGTACCAAACATTGATCATG | |
GSP2 | TTTGCCCTCATCAATTCCTCCGC | GCACTTGGAGACCCTCCTCAG | |
NGSP2 | CTCCAAGTGTTTCCAAGTACTCG | GGACCCCAACAGTGCGAGATC | |
R2d2 | GSP | CTTGAAGGTTCTTCAACCTTGTCAAC | GAGCCTACTGAGGCCTTACCCA |
NGSP | CTGACAGTAAACAATTTAGCATGGG | ATTCTGTTGGTGCATTAACTGAGTTC | |
Argonaute 2 | GSP | TTCTGGTCCATTGTCCGGACGG | GAACTCAAGAACGACTGGGCAGC |
NGSP | TGTCCCTTGGATTGTTCAATCTGC | GCTGCGCCGTTAGTTTCTGGTC | |
Sid 1 | GSP | GTATATTATTGCCATGTTATCCATGC | GTCCTCTTGAAAGAATGAAATCCTAG |
NGSP | ACTTATCTAGTCCTTGCTTGTGTG | GTGGAGTGCAGTGAAAACAACGTAG |