FIG. 2.
Gel filtration chromatography of purified SIVgp140-ATC(W) and SIV[ΔV1V2V3]-ATC expressed from insect cells. The chimeric proteins were purified by MAb 17A11 affinity chromatography from supernatants of insect cell cultures infected with recombinant baculoviruses expressing SIV gp140-ATC(W) (A) and SIV[ΔV1V2V3]-ATC (B). The purified proteins were then resolved by gel filtration chromatography using a Superose 6 column. Molecular masses were calculated based on a standard curve plotted using known proteins, which included thyroglobulin (669 kDa), ferritin (440 kDa), catalase (232 kDa), aldolase (158 kDa), and bovine serum albumin (67 kDa). Peak fractions were pooled and analyzed by SDS-PAGE (inset).