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. 2013 Dec 31;8(12):e83832. doi: 10.1371/journal.pone.0083832

Figure 1. In vitro sensitivity of Metformin in sarcomas.

Figure 1

A) Cell growth was assessed by MTT assay after 72h exposure to Metformin (mM) in seven Ewing sarcoma, four Osteosarcoma, and three Rhabdomyosarcoma cell lines and displayed as a percentage of survival over controls. Points, mean of two independent experiments; bars, SE. B) Effects of metformin on sarcoma cells in anchorage independent conditions. Number of colonies was determined after 7–10 days of growth in IMDM 10% FBS. Columns, mean of three independent experiments; bars, SE. Bars: mean of three experiments ± SE. *P < 0.05, ** P<0.01, ***P < 0.005 statistically significant differences by Student’s t test. Representative pictures of the effects of the inhibitor on colonies formation in soft agar. Magnification x40; C) IC50 values of metformin in Ewing sarcoma (TC/Doxo8) and osteosarcoma (U-2OS/Doxo35) chemoresistant cell lines.