Skip to main content
. 2013 Dec 31;8(12):e83937. doi: 10.1371/journal.pone.0083937

Figure 1. CHH gene map based on GenBank sequence AF474409.

Figure 1

1. The position of the exons, introns and the PCR primers within the sequence are marked. The expected amplicon expands between exon 2 and 4. B. The primer position is shown (underlined base pairs) over the eyestalk CHH mRNA precursor sequence (Gene Bank acc. no. AB027291.1). Bold letters represent the coding region of the CHH protein. C. Base pair sequence alignment between the eyestalk CHH mRNA precursor (Eyestalk-CHH) and the amplicon sequences obtained in this work: the optic lobe sequence, PcOL-CHH, the brain sequence, PcB-CHH and the brain CHH-like sequence, CG-CHHL (GenBank acc. no. AY256877.1). D. The amino acid sequence alignments between Eyestalk-CHH, the deduced amino acid sequence from the optic lobe and brain amplicons obtained in this work, PcOL-CHH and PcB-CHH, the CHH-like sequence from the thoracic ganglia (1–5 TG-CHHLs; GenBank acc. nos. AAL79193.1, AF474408.1, JF311403, AY256876.1, JX856149) and the deduced amino acid sequence from CG-CHHL.