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. 2013 Dec 31;8(12):e83833. doi: 10.1371/journal.pone.0083833

Figure 4. Stress-related, serum-free conditions increases BDNF and NT-3 expression in UCB NCs and lineage-negative cells.

Figure 4

(A) QRT-PCR shows changes in mRNA expression of BDNF and NT-3 in NCs after 24, 48, and 72 h in serum-free media. Relative mRNA expression levels are normalized against BMG. Data are shown as median, quartiles, interquartile range, minimum, and maximum for twelve independent experiments. *P<0.05 versus 0 h. (B) QRT-PCR shows changes in the mRNA expression of BDNF and NT-3 in lineage-negative cells after 24, 48, and 72 h in serum-free media. Relative mRNA expression levels are normalized to BMG levels. Data are shown as median, quartiles, interquartile range, minimum, and maximum for six independent experiments. *P<0.05 versus 0 h. (C) Representative western blot analysis of BDNF and NT-3 protein levels in NC lysates. (D) Representative western blot analysis of BDNF and NT-3 protein levels in lineage-negative cell lysates.