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. 2004 May;78(9):4665–4674. doi: 10.1128/JVI.78.9.4665-4674.2004

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FIG. 1. — Map of pFab CMV dhfr vector for expression of full-length IgG1 in CHO cells and structure of the IgG1 light-chain and heavy-chain DNA inserts. (A) Locations of the various genes present in the expression vector. LC, light-chain DNA; pA, poly(A) addition signal; HC, heavy-chain DNA. The arrows indicate transcription direction. (B) Structure of the humanized IgG1 light-chain and heavy-chain genes under the control of an hCMV early promoter. V_L and C_L are the light-chain hypervariable region and constant region, respectively. V_H, heavy-chain hypervariable region; C_H1, constant region 1; hg, hinge; int-1, intron 1 (118 nucleotides); C_H2, constant region 2; int-2, intron 2 (97 nucleotides); C_H3, constant region 3. The dark-shaded regions are human IgG1 sequences, and the medium-shaded regions represent chimpanzee IgG1 sequences. The selectable neo and dhfr genes (light shaded) are flanked by a β-globin promoter (β-glo) and a poly(A) addition site (pA).

FIG. 1. — Map of pFab CMV dhfr vector for expression of full-length IgG1 in CHO cells and structure of the IgG1 light-chain and heavy-chain DNA inserts. (A) Locations of the various genes present in the expression vector. LC, light-chain DNA; pA, poly(A) addition signal; HC, heavy-chain DNA. The arrows indicate transcription direction. (B) Structure of the humanized IgG1 light-chain and heavy-chain genes under the control of an hCMV early promoter. V_L and C_L are the light-chain hypervariable region and constant region, respectively. V_H, heavy-chain hypervariable region; C_H1, constant region 1; hg, hinge; int-1, intron 1 (118 nucleotides); C_H2, constant region 2; int-2, intron 2 (97 nucleotides); C_H3, constant region 3. The dark-shaded regions are human IgG1 sequences, and the medium-shaded regions represent chimpanzee IgG1 sequences. The selectable neo and dhfr genes (light shaded) are flanked by a β-globin promoter (β-glo) and a poly(A) addition site (pA).