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Analysis of antigenic specificity by radioimmunoprecipitation. (A) [35S]methionine-labeled lysates of DENV-4-infected Vero cells were precipitated with the various Fab preparations indicated. (B) [35S]methionine-labeled lysates were prepared from CV-1 cells infected with vaccinia virus recombinant containing the full-length coding sequence of vDEN-4 prM or E, respectively. E+prM, precipitations with a mixture of both lysates; HMAF, precipitation using HMAF raised against DENV-4.
