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. 2004 May;78(9):4665–4674. doi: 10.1128/JVI.78.9.4665-4674.2004

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FIG. 4. — Epitope analysis of chimpanzee Fab antibodies against DENV-4 by competition ELISA. Selected Fabs were affinity purified, biotinylated, and used for the analysis of binding reactivity to DENV-4 virions by competition ELISA in the presence of competing, unlabeled Fabs. (A) Biotinylated Fab 3C1; (B) biotinylated Fab 3E4; (C) biotinylated Fab 7G4; (D) biotinylated Fab 5H2. Chimpanzee Fab 1F2, which did not bind to DENV-4, was used as a negative control. The numbers on the y axis are OD readings, and the x coordinate represents reciprocal dilutions of the competing Fabs.