TABLE 3.
Binding activities of Fab monoclonal antibodies to DENV-4 and other dengue virus serotypes as determined by ELISAa
| Fab | ELISA titer of Fab binding to
|
|||
|---|---|---|---|---|
| DENV-4 | DENV-1 | DENV-2 | DENV-3 | |
| 5A7 | 3.41 | 2.51 | 2.51 | 2.51 |
| 3C1 | 3.71 | 1.30 | 3.11 | 1.30 |
| 3E4 | 4.61 | 4.61 | 4.31 | 4.61 |
| 7G4 | 4.01 | 4.01 | 4.01 | 4.01 |
| 5D9 | 3.41 | <1.0 | <1.0 | <1.0 |
| 5H2 | 4.01 | <1.0 | <1.0 | <1.0 |
| 1F2b | 1.30 | <1.0 | <1.0 | <1.0 |
a
Microtiter plates were coated with DENV-1, DENV-2, DENV-3, or DENV-4 virions. The starting amount of each Fab in ELISA was approximately 300 μg/ml. Data are presented as log10 of the reciprocal dilution that gave an OD reading of twofold greater or higher than the background.
b
Chimpanzee Fab from library D4 was used as the negative control for binding to DENV-4 and other dengue virus serotypes.