Figure 5. Metal analysis of different leaves in wild type and osnramp3 plants.

Different leaves were sampled from wild type and osnramp3 plants grown for two weeks at 0.08 (A) or 8 (B) µM Mn supplies after two weeks of normal conditional cultivation by hydroponics and elemental analysis was performed by ICP-MS. The 1–4 leaves were harvested from the same tiller of wild type or osnramp3 plants, and represented leaves from oldest to youngest respectively. Data are means ± SD (n = 3). One and two asterisks indicate values are significantly different from wild type at P<0.05 and P<0.01, respectively (by t-test).