Figure 3. Peritubular capillaries (PTC) and macrophage infiltration in unilateral ureteral obstruction (UUO).

Confocal microscopy identifies CD31⁺ (green) PTC endothelial cells in wild type (WT, A&C)) and NOS3 knockout (KO, B&D) mouse kidneys 7 days after sham (A&B) or UUO surgery (C&D). In UUO kidney, PTCs appear compressed and misshapen (C&D). Quantification of CD31+ PTC lumina/mm² (E) and F4/80+ macrophages infiltration (F). Data are mean ±SD from groups of 5 mice and analysis by one-way ANOVA with post hoc analysis with Tukey's multiple comparison test. ***p<0.001 versus sham operated group. #p<0.05 versus WT operated group. Magnification, ×600 in A to D.