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. 2013 Dec 31;8(12):e84063. doi: 10.1371/journal.pone.0084063

Figure 9. L-NAME-treated media induces a pro-fibrotic response in renal fibroblasts (NRK49F cells).

Figure 9

(A) Western blot showing expression of fibronectin, α-smooth muscle actin (α-SMA), collagen I and GAPDH in NRK49F cells cultured with L-NAME-treated or control MMEC media in the presence or absence of TGF-β1 stimulation for 24 hrs. (B) Quantification of the relative expression of fibronectin, α-SMA and collagen I compared to GAPDH. Data are mean ±SD and analysis by one-way ANOVA with post hoc analysis with Tukey's multiple comparison test. *p<0.05, **p<0.01. Experiments were repeated at least three times.