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. 2013 Dec 31;8(12):e84063. doi: 10.1371/journal.pone.0084063

Figure 10. L-NAME-treated media enhances PDGF-BB-induced proliferation of renal fibroblasts (NRK49F cells).

Figure 10

Sub-confluent NRK49F cells were incubated in L-NAME-treated or control MMEC media in the presence or absence of PDGF-BB stimulation for 24 hr. (A) Immunostaining of Ki67 (brown) with nuclear staining (blue) in NRK49F cells. (B) Quantification of the percentage of NRK49F cells positive for Ki67 immunostaining. (C) Quantification of cell proliferation using incorporation of bromodeoxyuridine (Brdu). Data are mean ±SD and analysis by one-way ANOVA with post hoc analysis with Tukey's multiple comparison test. **p<0.01, ***p<0.001. Experiments were repeated at least three times.