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. 2013 Dec 31;8(12):e84063. doi: 10.1371/journal.pone.0084063

Figure 11. L-NAME-treated media induces phosphorylation of JNK and the Smad3 linker region in renal fibroblasts (NRK49F cells) which is inhibited by RvD1.

Figure 11

(A) Western blot (WB) showing phosphorylation of JNK and p-S208 in the Smad3 linker region in NRK49F cells incubated with L-NAME conditioned MMEC media which is inhibited by RvD1. (B) Immunoprecipitation (IP) of Smad3 followed by WB identified phosphorylation of the Smad3 C-terminal domain (p-C-Smad3) and linker region (p-T179) following culture of NRK49F cells in L-NAME conditioned MMEC media which is inhibited by RvD1. (C) Quantification of phosphorylation of JNK and the different sites on Smad3. Data are mean ±SD and analysis by one-way ANOVA with post hoc analysis with Tukey's multiple comparison test. *p<0.05, ***p<0.001, N.S., not significant. Experiments were repeated at least three times.