Figure 13. Regulation of collagen I promoter activity.

(A) NRK49F cells were transfected with a reporter plasmid in which luciferase production is driven by the collagen I promoter. Incubation of these cells with L-NAME treated MMEC media for 24 hr up-regulated collagen I promoter activity which was prevented by the addition of RvD1. (B) 293T cells were transfected with Smad3 plasmids containing point mutations of various phosphorylation sites plus a collagen I promoter plasmid. Twenty-four hours later, cells were cultured with or without TGF-β1 and luciferase activity measured 24 hr later. Data are mean ±SD and analysis by one-way ANOVA with post hoc analysis with Tukey's multiple comparison test. * p<0.05, ***p<0.001, N.S., not significant. Experiments were repeated at least three times.