FIG. 1.

Growth arrest of A549 cells during infection with MeV strain Hälle. (A) Growth of A549 cells under normal conditions (control), infected with MeV (MOI, 1) (MeV), or treated with IFN-γ (1,000 U/ml) (IFN-γ), as determined by cell numbers. Cell numbers were determined by the gentian violet dye binding assay. The error bars represent standard deviations. (B) Cell cycle analysis by flow cytometry of A549 cells infected with MeV after propidium iodide staining. mock, uninfected control; d, day(s). (C) Western blotting analysis of Rb protein and cyclin E in A549 cells infected with MeV. pRb and p-pRb indicate unphosphorylated and phosphorylated forms of Rb protein, respectively. Actin was used as a control for protein loading.