FIG. 5.

Phosphorylation status of Jak1 and STAT-1 in SiHa cells persistently infected with various strains of MeV. Cell lysates from SiHa cells and SiHa cells infected with various MeV strains were applied to Western blotting using antibodies specific for the phosphorylated form of Jak1 or STAT-1. Each cell line was treated with IFN-γ (1,000 U/ml for 15 min) (+) as a positive control for phosphorylation of Jak1 and STAT-1. The protein levels of total Jak1 and STAT-1 were determined as a control for protein loading. Constitutive Jak1 phosphorylation in MeV-infected cells was determined and expressed as a percentage relative to the value obtained from the same cell line treated with IFN-γ.