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. Author manuscript; available in PMC: 2014 Dec 26.
Published in final edited form as: Mol Cell. 2013 Nov 21;52(6):10.1016/j.molcel.2013.10.020. doi: 10.1016/j.molcel.2013.10.020

Figure 4. Primase Concentration Influences the Rate of Leading-strand Reinitiation.

Figure 4

(A) Illustration of the replication products generated following restarted replication, or complete uncoupled replication downstream from the CPD.

(B) Titration of DnaG using standard reaction conditions for 6 min.

(C) Quantification of full-length replication products (restarted leading strands and lagging strands) from pulse-chase experiments conducted using 200 nM and 800 nM DnaG on the CPD-A+C template. Note that complete uncoupled replication is not observed at either concentration of DnaG (Figure S6). Error bars represent the SEM from three independent experiments.