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. Author manuscript; available in PMC: 2014 Dec 26.
Published in final edited form as: Mol Cell. 2013 Nov 21;52(6):10.1016/j.molcel.2013.10.020. doi: 10.1016/j.molcel.2013.10.020

Figure 5. The DnaX Complex Enhances the Efficiency By Which Leading-strand Synthesis is Reinitiated Downstream of a Lesion.

Figure 5

(A) DnaG titration following column isolation of ERIs. Reactions were conducted for 6 min on the CPD-A template.

(B) Column-isolated replication reactions using the CPD-A template and 1 μM DnaG in the presence or absence of DnaX-γ3 complex that was added 45 sec post EcoRI.

(C) Column-isolated replication reactions conducted at 250 nM DnaG with the CPD-A template. DnaX-γ3 complexes (2.5 nM) were added at different time points following EcoRI addition.

* ERIs that are labeled but not extended.