Figure 3. Effect of the F11L gene on MYXV growth in human cancer cells.

(A) MYXV yields on different cell lines. The cells indicated were infected with WT, ΔM127L-mCh, or F11L-mCh viruses at MOI = 0.01. The viruses were harvested 72 h later, and titered on BGMK cells. For each cell and virus, we report the virus yield measured relative to the amount of input virus from three independent experiments (mean±S.E.M.) (B) MYXV plaque production on different cancer cells. The 11 different cell lines were infected with the indicated MYXV strains at MOI = 0.1. The cells were fixed four days later and the virus-encoded β-galactosidase activity detected using X-Gal. BGMK is a monkey cell line, the rest are of human origin.