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. 2013 Dec 31;8(12):e84116. doi: 10.1371/journal.pone.0084116

Figure 7. Apoptosis assessed by annexin V/propidium iodide (PI) assay.

Figure 7

Annexin V/PI staining was performed to assess the apoptosis rate in microglia. After incubating in DMEM/F-12 or BMSC-CM with or without LPS (1 µg/mL) for 48 hours, microglia were harvested. Cells were then stained with annexin V-FITC and propidium iodide and analyzed by flow cytometry. The results are presented as density plots of propidium iodide vs. annexin V-FITC. Apoptotic cells have high annexin V-FITC and low propidium iodide staining (lower-right quadrant). Numbers on the graph represent the percentages of apoptotic cells in the different groups.