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. 2013 Dec 31;8(12):e84191. doi: 10.1371/journal.pone.0084191

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© 2013 Owczarek et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunohistochemical staining with monoclonal antibody against Ki67 antigen of tumor sections after subcutaneous implantation of control MDA/LUC cells (I) and sh-transduced MDA/LUC-shUGT8 cells with decreased expression of UGT8 (II) into nu/nu mice. The numbers of Ki67-positive cells in MDA/LUC (I) and sh-transduced MDA/LUC-shUGT8 tumors were compared by Mann-Whitney U-test (^***p<0.0001). (B) TUNEL technique after subcutaneous implantation of MDA-M/LUC cells (I) and MDA/LUC-shUGT8 cells (II) breast cancer cells into nu/nu mice. Original magnification: x100. The numbers of apoptotic cells in MDA/LUC (I) and sh-transduced MDA/LUC-shUGT8 tumors were compared by Mann-Whitney U-test (^*p = 0.0432).