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. 2013 Dec 31;8(12):e84112. doi: 10.1371/journal.pone.0084112

Figure 2. SDS-PAGE and Western blot analysis during OCX-36 purification.

Figure 2

OCX-36 protein was extracted with buffer E (50 mM Tris-HCl, pH 8.5) and purified by CM-Sepharose Fast Flow and DEAE-Sepharose Fast Flow chromatographies. (A) 12.5% SDS-PAGE gel and (B) Western blot of OCX-36 fraction collected from each step of purification. The position of molecular weight standards (KDa) is indicated. Lane 1: Supernatant from overnight extraction prior to chromatography; Lane 2: Flow-through unbound to DEAE Sepharose; Lane 3: Sample prepared from DEAE beads before elution; Lanes 4–7: Fractions 1, 2, 3 and 4 eluted from the DEAE Sepharose with 50 mM Na Hepes, pH 7.0, 2 mM DTT, 350 mM NaCl; Lane 8: Sample prepared from DEAE beads after elution. Position of OCX-36 (Ovocalyxin-36) indicated by the arrow.