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. 2013 Dec 31;8(12):e84112. doi: 10.1371/journal.pone.0084112

Figure 9. LPS and LTA binding activity of OCX-36 (mixture) and OCX-36 individual forms (Pro-71 and Ser-71).

Figure 9

The LPS and LTA binding to OCX-36 (mixture), OCX-36 (Pro-71) and OCX-36 (Ser-71) were tested with the ELISA binding assay. Binding activity was corrected for background BSA binding and individual experiments were normalized before merging. (A)LPS-BINDING: All proteins tested showed E. coli LPS binding activity at 1.5, 5, 15 and 50 µg/mL but a significant difference was not observed between them at any concentration. All concentrations of Pro-71 showed significant different LPS binding activity between them (p<0.05). (B) LTA-BINDING: The LTA binding activity of OCX-36, Pro-71 and Ser-71 were tested at 1.5, 5 and 15 µg/mL. The LTA binding activity of OCX-36 (15 µg/mL) and Ser-71 (5 and 15 µg/mL) were significantly different than the Pro-71 (*p<0.05). All concentrations of Pro-71 showed LTA binding activity significant difference between them (p<0.05). The LTA binding activity of OCX-36 at 5 and 15 ug/mL were significant different between these concentrations (p<0.05). The results are the average of three individual experiments, with each experiment performed in triplicate.