TABLE 11.

Protection of monkeys against challenge with WT DEN1 to DEN4 viruses in cynomolgus monkeys immunized s.c. (∼ 6 months previously) with one dose of ChimeriVax-DEN1-4 GMP vaccine formulation

Group	Previous CV DEN1-4	Challenge virusa	Strain of challenge DEN virus	No. viremic/ total no.	Mean viremia		% protectedb	GMT PRNT50
					Peak (log10 PFU)	Duration (days)		Prechallenge	Postchallenge
5	Yes	DEN1	West Pacific 74	1/6	1.7 (3.3)c	0.7 (4.0)c	83	63	14,481
6	No	DEN1		4/4	3.2	6.0	0	<10	3,044
7	Yes	DEN2	S16803	0/6			100	30	1,437
8	No	DEN2	PDK-10	4/4	2.5	4.5	0	<10	538
9	Yes	DEN3	CH53489	0/6			100	718	10,240
10	No	DEN3		4/4	2.2	4.2	0	<10	2,229
11	Yes	DEN4	341750 Caribbean	1/6	0.6 (1.7)c	0.3 (2)c	83	127	2,032
12	No	DEN4		4/4	3.2	5.2	0	<10	3,620

^aTwo animals in each of groups 6, 8, 10, and 12 received 4 log units of challenge virus (in the pilot study); all other animals received 5 log units of appropriate challenge virus (main study).

^bNo virus could be found in a plaque assay in Vero cells when using undiluted or 1:2 or 1:10 dilutions of sera obtained from days 2 to 11 postchallenge.

^cViremic animals only.