Table 2. Reproductive performance of cattle clones and their genetic donors after AI and IVF.
Superovulation with AI | Ovum pickup with IVF | |||||||
Breeding group | N | Age | Flushed Embryos | Transferable Embryos | N | Age | Oocytes | Transferable Embryos |
Genetic donor (Average ± SD) | 34 | 6.65±3.20 | 10.47±4.75 | 4.77±3.15 | 25 | 9.32±4.04 | 17.7±8.14 | 5.09±4.39 |
Clone (Average ± SD) | 85 | 3.37±1.30 | 9.68±6.57 | 4.42±3.38 | 61 | 3.90±1.58 | 18.9±9.00 | 6.04±5.85 |
Equal variance test (P value)a | <0.0001 | 0.0391 | 0.6589 | <0.0001 | 0.5963 | 0.1205 | ||
Minimum differenceb | 3.07 | 1.86 | 5.67 | 3.27 |
Notes: Values shown are the number of animals (N) per breeding group or the average values for age, number of flushed embryos, number of transferable embryos or number of oocytes ± standard deviation for each breeding group.
a The SAS ttest procedure was used to compare variances between genetic donor and clone breeding groups. Results of the test of equal variances (method: folded F) are shown for each comparison category.
b Retrospective power analyses were performed (t-test with two-tailed α = 0.05) to determine the minimum significant difference detectable (at 80% power) for all comparisons between genetic donor and clone shown above (except age). For example, with N = 34 for donors (σ = 4.75) and N = 85 for clones (σ = 6.57), we would have 80% power to detect a significant difference of 3.07 flushed embryos generated by superovulation with AI.